Effects of SYUIQ-5,a novel antitumor agent,on gene expression of hepatic cytochrome P-450 in rats
- VernacularTitle:抗肿瘤活性物SYUIQ-5对大鼠肝组织细胞色素P450基因表达的影响
- Author:
Qibiao SU
;
Fan HE
;
Yujing LU
;
Lianquan GU
;
Min HUANG
- Publication Type:Journal Article
- Keywords:
Cytochrome P450 enzyme system;
Enzyme inhibitors;
Reverse transcriptase polymerase chain reaction;
Animal experimentation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of SYUIQ-5,a novel antitumor agent,on gene expression of hepatic cytochrome P450 in rats.Methods Male Sprague-Dawley rats were administered daily intraperitoneal dose of SYUIQ-5(0.1mg/kg,5mg/kg and 10mg/kg body weight) for 5 consecutive days.The levels of CYP 3A1,2B1/2,1A1,1A2 and 2E1 mRNA in rat liver were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).Results The level of gene expression of CYP 1A1 was increased significantly compared with the control.The levels of CYP 3A1,2B1/2,1A2 and 2E1 mRNA were not changed by SYUIQ-5 treatment compared with the control.Conclusion A significant increase in the CYP 1A1 rather than CYP3A1,2B1/2,1A2 and 2E1 gene expression by SYUIQ-5 treatment is observed.
