Effects of ketamine on expression of nitric oxide synthase in medial septal nuclei and vertical diagonal band of rats
- VernacularTitle:氯胺酮对大鼠隔内侧核和斜角带核垂直支一氧化氮合酶表达的影响
- Author:
Feng WU
;
Keren XIONG
- Publication Type:Journal Article
- Keywords:
ketamine;
medial septal nuclei;
diagonal band;
nitric oxide synthasdyeing;
rats
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects in different dosage of ketamine on the expression of nitric oxide synthase (NOS) in medial septal nuclei (MS) and vertical diagonal band (VDB) of rats. METHODS: 40 SD rats were randomized into normal control group,normal saline(NS) group, low, middle and high dosage of ketamine(25,50,100 mg?kg~ -1 ,ip) group.Each group was maintained intraperitoneally by given daily dosage once a day for a period up to 7 days. That was done at 2100 hour each night, and by 2 h of the last administration, poured, fixed and sliced were carried out. Method of nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) was used to show the expression of NOS in MS and VDB pars dorsalis and ventro. RESULTS: Compared with normal control and NS group, the number of NOS positive neurons were decreased in MS and VDB pars dorsalis and ventro in ketamine groups.There were statistical meaing in different-dosage groups of ketamine(P
