Effects of hirudo and earthworm liquor extract on syndecan-1 in rats with experimental cerebral ischemia reperfusion
- VernacularTitle:水蛭地龙萃取液对大鼠局灶性脑缺血再灌注损伤粘结合蛋白多糖-1的影响
- Author:
Haiyan LUO
;
Changlin HU
- Publication Type:Journal Article
- Keywords:
hirudo and earthworm liquor extract;
syndecan-1;
cerebral ischemia;
reperfusion damage
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of hirudo and earthworm liquor extract on the expression of syndecan-1 in focal cerebral ischemia reperfusion rats and the relationship between syndecan-1 expression and cellists underlying antiinflammatory mechanism. METHODS: The MCAO models of rats were built with the intraluminal filament occlusion. Rats' brains were cut at the levels of the hippocampus as the templates. Immunohistochemistry staining and HE staining were used to facilitate the observation of the distribution and the quantities of cells with expression of syndecan-1 and the pathological change of MCAO ischemia 2 h reperfusion 4, 24, 72 h and 7 d with hirudo and earthworm liquor extract or saline treatment, respectively. RESULTS: Hirudo and earthworm liquor extract could upregulate the expression of syndecan-1 on ischemia/ reperfusion in rats' brains, especially at 72 h. The numbers of syndecan-1 cells got peak, hirudo and earthworm extract liquor could downregulate polynucleation inflammatory cells at 24 and 72 h. The expression of inflammatory cells in both hirudo and earthworm liquor extract group and saline group at 4 h was not significant. Hirudo and earthworm extract liquor downregulated the monocytic inflammation cells infiltration at 7 d. CONCLUSION: Hirudo and earthworm liquor extract can upregulate the expression of syndecan-1 on ischemia reperfusion in rats' brains. The upregulation of syndecan-1 can decrease inflammatory cell infiltrate.
