Effects of xy9902 on the proliferation and differentiation of MC3T3-E1 cells
- VernacularTitle:xy9902对MC3T3-E1细胞的增殖和分化作用
- Author:
Jin HOU
- Publication Type:Journal Article
- Keywords:
proliferation;
MC3T3-E1 cells;
ER;
differentiation
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of xy9902 on the proliferation and differentiation in MC3T3-E1 cells and its mechanism.Methods Effects of xy9902 on the proliferation and differentiation of MC3T3-E1 cells was determined by MTT and ALP activity measurement.Competitive estrogen-receptor binding assay was used to investigate the affinity of xy9902 to ER.Results xy9902 induced cell proliferation and increased alkaline phosphatase(ALP) activity of MC3T3-E1 cells.Antiestrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1 induced by xy9902.The relative binding affinity of xy9902 for ER? and ER? showed an IC_(50)of 8.45?10~(-6) mol?L~(-1) and 1.66?10~(-6) mol?L~(-1) respectively.Conclusion xy9902 directly stimulates cell proliferation and differentiation of MC3T3-E1 cells through ligand-receptor binding,which indicated that xy9902 behaves as an estrogen agonist in MC3T3-E1 cells.
