Effect of astragalosides on schistosomal hepatic fibrosis in vitro
- VernacularTitle:黄芪总苷对血吸虫卵抗原活化的肝星状细胞增殖与胶原合成的影响
- Author:
Hong ZHU
;
Qiang WU
;
Yan YANG
;
Xiaosong YUAN
;
Xuelong WANG
;
Jilong SHEN
- Publication Type:Journal Article
- Keywords:
astragalosides;
soluble egg antigen;
hepatic stellate cell;
proliferation;
collagen synthesis
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of astragalosides on proliferation and collagen synthesis of HSC-T6 cells driven using schistosoma japonicum soluble egg antigen-activated macrophage conditioned medium(SEA-MCM).Methods SEA-MCM was prepared by injection of Schistosoma japonicum SEA via mice peritoneal.The proliferation and collagen synthesis of HSC-T6 cells stimulated with SEA-MCM were measured using MTT colorimetric assay and()~3H-proline incorporation respectively.Results The proliferation and collagen production of HSC-T6 cells were significantly promoted by SEA-MCM.They were significantly suppressed after being treated with AST(32.5、65、130 mg?L~(-1)) showing concentration-dependent effect.Conclusion:Astragalosides may inhibit HSC-T6 cells proliferation and collagen production that was driven by SEA activated MCM in vitro.
