Effects of PGF2? on the glucose-stimulated insulin secretion in NIT-1 beta cells
- VernacularTitle:PGF_(2?)对NIT-1?细胞葡萄糖刺激性胰岛素分泌的影响
- Author:
Chunling YE
;
Zhenyu YUAN
;
Bing SHEN
;
Jianjun LIU
;
Chaoxia LU
- Publication Type:Journal Article
- Keywords:
prostaglandin F_(2?);
insulin secretion;
NIT-1 beta cell;
[Ca~(2+)]i
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of PGF_(2?) upon glucose-stimulated insulin secretion and the calcium response in NIT-1 beta cells.Methods Using the radioimmunoassay(RIA),the amount of PGF_(2?) augmentation of glucose-stimulated insulin secretion was determined in different conditions and the confocal laser scanning methods by Fluo-3AM as a fluorescent probe were used to analyze the NIT-1 beta cell intracellular calcium response in correlated various terms.Results In the presence of 16.5 mmol?L~(-1) glucose,PGF_(2?)(0.1,1,5 ?mol?L~(-1)) dose-dependently augmented glucose-induced insulin secretion in NIT-1 beta cells,especially at 5 ?mol?L~(-1)(P0.05).Meanwhile,Exposure of the NIT-1 cells to 5 ?mol?L~(-1) PGF_(2?) induced a rapid increase of intracellular calcium(P
