Protective effects of puerarin on rat primary cultured neurons damaged by glutamic acid
- VernacularTitle:葛根素对谷氨酸所致大鼠原代神经细胞损伤的保护作用
- Author:
Limin CHEN
- Publication Type:Journal Article
- Keywords:
puerarin;
primary cultured neurons;
membrane lipids fluidity;
fluorescence polarization;
1,6-Dipheyl-1,3,5-hexatriene
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effects of puerarin on the neurons damaged by glutamic acid.Method The rat primary cultured neurons were damaged by glutamic acid,the membrane lipids fluidity was studied with fluorescence polarization(P) and mean microviscosity(?) with DPH as a probe;the protective effects of puerarin on this model were evaluated by lactate dehydrogenase(LDH) efflux assay and colormetric MTT assay.Results The fluorescence polarizating and mean microviscosity of puerarin were lower than glutamic acid model group in a dose-depended manner.Treatment of puerarin resulted in the decrease of LDH release from primary cultured neurons damaged by glutamic acid and increase in the optical density(A) at 540 nm tested by colorimetric MTT assay.No proliferating effects on normal primary cultured neurons were observed.Conclusion The results suggested that puerarin had a protective effect on rat primary cultured neurons damaged by glutamic acid,it might be relative to the improvement of membrane lipids fluidity
