Establishment of a real-time fluorescence quantitative RT-PCR for measurement of t-PA mRNA expression in endothelial cells
- VernacularTitle:实时荧光定量RT-PCR检测内皮细胞t-PA mRNA方法的建立
- Author:
Yanting ZHAO
;
Lianfen ZHANG
;
Jian JIN
- Publication Type:Journal Article
- Keywords:
real-time RT-PCR;
tissue plasminogen activator;
human microvascular endothelial cells.
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
0.990).The intra-assay and inter-assay variation of the method was 3.10 % and 4.93 %,respectively.The all-trans ratinoic acid(ATRA) up-regulated t-PA mRNA expression in a dose-dependent manner(1.25~20.00 ?mol?L~(-1),P