ATRA combined with IFN? to induce differentiation and growth inhibition of NB cells.
- VernacularTitle:全反式维甲酸联合?-干扰素对神经母细胞瘤细胞诱导分化及生长抑制作用的研究
- Author:
Yanmin YANG
;
Yuqin CHE
;
Yan LU
- Publication Type:Journal Article
- Keywords:
Neuroblastoma;
ATRA;
IFN?;
Cell differentiation;
Growth inhibition
- From:
Chinese Journal of Practical Internal Medicine
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ATRA and IFN? on growth and differentiation of SH-SY5Y cell line and the possible molecular mechanisms.Methods The expression of TrkA mRNA was detected by RT-PCR.Trypan blue exclusion was used to detect the antiproliferative effect of ATRA,IFN?and ATRA +IFN? on SY5Y cells.Morphologic changes were observed using phase-contrast microscopy.Results TrkA mRNA was upregulated by ATRA and IFN?.ATRA and IFN? could induce morphological differentiation and inhibit growth of SY5Y cells.The combination of ATRA+IFN? enhanced the induced differentiation and growth inhibition of SY5Y cells.Conclusion Synergistic effects of ATRA and IFN? on growth inhibition and inducing differentiation in SY5Y cells are observed and this may result from upregulating the expression of TrkA mRNA.
