Investigation on the ESBLs Produced in the biofilm of bacteria.
- VernacularTitle:生物被膜肺炎克雷伯菌超广谱?-内酰胺酶的检测
- Author:
Naijing LI
;
Ping HE
;
Yanmin YANG
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Biofilm;
ESBLs
- From:
Chinese Journal of Practical Internal Medicine
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective evaluate the mechanism that bacteria growing in biofilm always resist to antimicrobial agents,and to provide the theoretical basis for selecting antimicrobial agents in clinic.Methods Model of klebsiella pneumoniae bacterial biofilm were built up with the modified flat-board method and identified with the method staining with AgNO3 and confocal scanning laser microscopy.We used imipenem to induce the ESBLs production of BF bacteria.ESBLs production was performed by the standard disk diffusion method.Results The isolation rate of ESBLs producting strains in klebsiella planktonically planktonically were 20.0%(8/40).The isolation rate of ESBLs producting strains in klebsiella biofilm were 42.5%(17/40).The isolation rate of ESBLs producting strains in klebsiella biofilm being induced by imipenem were 65.0%(26/40).The isolation rate of group A and group B,groupB and group C was different from each other significantly with the statistic method of ?2-test.Conclusion One of the main reasons that klebsiella and escherichia coli resist to antibiotics is the synergetic effect of BF and ESBLs.
