Preparation and controlled release of colon-targeted carboxymethylchitosan microspheres containing levofloxacin:an in vivo study
- VernacularTitle:自制左氧氟沙星羧甲基壳聚糖缓释微球体内结肠靶向释放的实验
- Author:
Yang LI
- Publication Type:Journal Article
- Keywords:
levofloxacin-carboxymethyl-chitosan;
microshperes;
colon
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare levofloxacin-carboxymethyl-chitosan(LVFX/CMC) microspheres and to study their colon-targeted release in rats.Methods: KromasilRKR100-5C18(250 mm?4.6 mm)was used as the analytical column and the temperature was maintained at 50℃.The mobile phase consisted of a mixture of acetonitrile:0.1% trifluoroacetic acid(2080) pumped at a flow rate of 1.0 ml/min,with ?_(ex)295 nm and ?_(em)495 nm.Sixty healthy male SD rats were randomized into 2 groups: to receive gastric lavage with normal LVFX(40 mg,control group) and LVFX/CMC microspheres(40 mg,treatment group).HPLC was used to detect the concentrations of LVFX in the stomach,intestine,cecum,colon and blood in rats in both groups.Results: LVFX was detectable 3 h after LVFX/CMC microsphere lavage.Five hours after lavage,the levels of LVFX in cecum and colon were respectively(3.394?0.197) mg and(1.873?0.216) mg in treatment group,and(0.489?0.123) mg and((0.078?0.002)) mg in control group(P
