Up-regulation of osteopontin gene in BALB/c 3T3 cells induced by tumor promoters of TPA,okadaic acid and cadmium chloride
- VernacularTitle:佛波酯等3种促癌物诱导BALB/c3T3细胞骨桥素基因表达升高
- Author:
Lin AO
- Publication Type:Journal Article
- Keywords:
osteopontin gene;
tumor promoter;
12-Otetradecanoylphorbol-13-acetate;
okadaic acid;
cadmium chloride
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of osteopontin (OPN) gene expression induced by tumor promoter of 12-O-tetradecanoylphorbol13-acetate(TPA),okadaic acid(OA) or cadmium chloride(CdCl_(2)).Methods The two-stage transformation test of BALB/c 3T3 cells was established with MNNG as initiator,TPA,OA or CdCl_(2) as promoter respectively.Mouse toxicology gene chip was used to detect the gene expression of BALB/c 3T3 cells transformed with TPA,OA or CdCl_(2) respectively,and the expression of OPN gene was validated by real-time RT-PCR assay.Results TPA,OA or CdCl_(2) could increase the transcriptional expression of OPN gene in BALB/c 3T3 cells.The expression of OPN gene was up-regulated in the eight transformed cell colonies induced by TPA,OA or CdCl_(2) respectively.Moreover,the up-regulation of OPN gene expression was confirmed by real-time RTPCR assay.Conclusion The up-regulation of OPN gene expression is closely related to cell transformation of BALB/c 3T3 cells induced by TPA,OA or CdCl_(2).
