Detection of ?-nerve growth factor secreted by hair follicle bulge cell cultured in vitro
- VernacularTitle:体外培养条件下毛囊bulge细胞分泌的?神经生长因子的检测
- Author:
Sen LIN
- Publication Type:Journal Article
- Keywords:
hair follicle;
?-NGF;
bulge cells
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To detect ?-nerve growth factor(?NGF) secreted by hair follicle bulge cells cultured in vitro qualitatively and quantitively and search the relationship between ?-NGF and bulge cells growth condition.Methods The primary tissues from the labial part and around the barbell in inbred Wistar rats aged 6-8 d were stripped by micromanipulative technique and cultured.The ultrastructure of primary bulge cells was observed under transmission electron microscope(TEM).The secretion of ?-NGF was determined by ELISA and immunocytochemistry.Results Primary bulge cells were cultured in vitro successfully.?-NGF was strongly expressed in the plasma of cultured bulge cells detected by ICC.The secretion of NGF detected by ELISA was regularly correlated with the characteristic of primary cultured bulge cells.Conclusion Primary bulge cells secreted the highest ?-NGF when bulge cells grew into peak phase.The expression of ?-NGF must have some necessary relationships with hair follicle bulge cells.
