Photochemical effect increases gene transfection of EGFP plasmids mediated by polycation
- VernacularTitle:光化学作用增强阳离子聚合物介导EGFP转染结肠癌
- Author:
Weidong XIAO
;
Wei CHEN
;
Zulin CHEN
;
Jia LIU
;
Haiyan GE
- Publication Type:Journal Article
- Keywords:
photosensitizer;
photodynamic therapy;
polycation;
transfection
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of photochemical gene transfection on colon cancer cells mediated by polycation.Methods Based on SW480 and HT29 colon carcinoma cell culture,the intracellular localization of meso-tetraphenylporphine disulfonate(TPPS_(2a)) were observed by laser confocal scanning microscopy(LCSM).Photochemically induced cytotoxicity of TPPS_(2a) on SW480 and HT29 cell lines was evaluated by MTT assay.By flow cytometry,the light-induced transfection of EGFP plasmids mediated by PEI was studied.Results TPPS_(2a) exhibited typical vesicular localization in two colon carcinoma cell lines.The photochemical cytotoxicity based on TPPS_(2a) increased with light dose.The light dose of IC_(50) was 22 mW/cm~(2) for 6 min for SW480 cells and for 12 min for HT29 cells(wavelength: 405 nm).The light treatment based on TPPS_(2a) induced an increase in transfection efficiency to(29.61?1.15) for SW480 cells and(23.47?0.62) for HT29 cells(P
