Effect of antisense vimentin retrovirus on injured astrocytes
- VernacularTitle:反义波形蛋白逆转录病毒对损伤的星形胶质细胞的作用
- Author:
Yongzhi XIA
;
Jiangkai LIN
;
Hua FENG
- Publication Type:Journal Article
- Keywords:
astrocyte;
vimentin;
RT-PCR;
Western blotting
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of recombinant antisense Vim retrovirus on Vim gene expression of injured astrocytes(AST).Methods The injured AST in vitro was established and divided into two groups: experiment and control.After transfected with retrovirus carrying antisense complementary DNA(cDNA) for vimentin,vimentin gene expression of injured astrocytes was investigated by immunocytofluoresence,RT-PCR and Western blotting.Results The processes of the injured astrocytes transfected by retrovirus became retracted.Transfection of antisense vimentin retrovirus into injured astrocytes also inhibited the growth of astrocytes and decreased the expression of vimentin at both transcription and translation level.Conclusion Retrovirus carrying vimentin antisense cDNA could inhibit effectively the growth and vimentin gene expression of injured astrocytes in vitro.
