Protective effects of complementary peptide of anaphylatoxin C5a on lung injury of experimental sepsis mouse
- VernacularTitle:C5a反义肽对实验性脓毒症小鼠肺损伤的作用研究
- Author:
Baosheng LI
- Publication Type:Journal Article
- Keywords:
anaphylatoxin C5a;
complementary peptide;
sepsis;
lung
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effects of the antisense peptide of C5a(NH_(2)-EARLQRVFLYVNPS-COOH) on lung injury of mouse sepsis model by cecal ligation and puncture(CLP).Methods Mice were subjected to cecal ligation and puncture(CLP).Forty mice received 0.5 mg/ml antisense peptide of C5a at the dose of 1,2,4,8 mg/kg through the tail vein after CLP,with ten mice receiving physiological saline as control and the survival rate was observed.The pathological change of the lung and the arterial partial pressure of oxygen,lung wet weight/dry weight and myeloperoxidase(MPO) were determined in normal controls,sham operation mice,CLP mice and CLP mice recceiving antisense peptide at dose of 4 mg/kg(n=10 in each group).Results The mice receiving antisense peptide at dose of over 2 mg/kg had higher 96-hour survival rate(P
