Protective effects of olanzapine against PC12 cell apoptosis induced by ?-amyloid peptide 25-35
- VernacularTitle:奥氮平对淀粉样?蛋白_(25-35)诱导的PC12细胞凋亡的保护作用
- Author:
Zhenfu WANG
;
Zelan WEI
;
Xinmin LI
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
amyloid beta-protein;
PC12 cells;
olanzapine;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the protective effects of olanzapine against apoptosis of PC12 cells induced by ?-amyloid peptide 25-35 (A?_ 25-35 ). Methods Based on the model of apoptosis of PC12 cells induced by A?_ 25-35 , cell viability was determined by MTT assay. The expressions of Bax, Caspase-3 of PC12 cells induced by A?_ 25-35 and olanzapine were assessed by Western blot. Results 10 -14 -10 -5 mol/L A?_ 25-35 lowered the cell viability of PC12 cells, 50?mol/L and 100?mol/L olanzapine pretreatment enhanced the cell viability of PC12 cells, and there was significant difference compared with olanzapine non-pretreated groups (P
