Studies on the inhibitory effect of PTEN protein on the proliferation of rat renal fibroblasts stimulated by TGF-?_1
- VernacularTitle:PTEN编码蛋白对TGF-?_1刺激大鼠肾成纤维细胞增殖的抑制作用
- Author:
Yue CHENG
;
Furong LI
;
Fahuan YUAN
- Publication Type:Journal Article
- Keywords:
PTEN;
transforming growth factor beta;
fibroblast;
kidney
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PTEN protein on the proliferation of rat renal fibroblasts resulted from the stimulation of TGF-?_1. Methods In vitro, the cultured rat renal fibroblasts were transfected with the adenovirus containing PTEN. Invert fluorescent microscope was used to detect the GFP expression, meanwhile PTEN mRNA was determined by RT-PCR method. 48h after the transfection, TGF-?_1 was added into the culture medium in a concentration of 10ng/ml. After another 24h, the proliferation rate of rat renal fibroblasts was determined by means of MTT method. Immunocytochemistry was also used to detect the expression of PCNA. Results As GFP positive fibroblasts were detected,the expression of PTEN mRNA was significantly increased in the rat renal fibroblasts which were transfected with the adenovirus containing PTEN. Accompanied with the expression of PTEN, the proliferation of cultured rat renal fibroblasts resulted from the stimulation of TGF-?_1 was obviously inhibited, and the expression of PCNA was significantly decreased. Conclusion PTEN could suppress the proliferation of rat renal fibroblasts caused by stimulation of TGF-?_1, and it might be useful to inhibit renal fibrosis and delay the rate of renal impairment.
