Expression of phosphoinositide 3 kinase in rats with acute lung injury induced by oleic acid
- VernacularTitle:磷脂酰肌醇3-激酶在油酸型急性肺损伤大鼠肺内的表达及其意义
- Author:
Yan LIN
;
Yan LIU
;
Senyang YU
- Publication Type:Journal Article
- Keywords:
phosphatidylinositol 3-kinase;
acute lung injury;
apoptosis;
signal transduction
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of phosphoinositide 3 kinase (PI3-K) and its relationship with lung cell apoptosis in rats with acute lung injury induced by oleic acid. Methods 60 healthy male SD rats were divided into 5 groups randomly, with 12 rats for each group. 0.25ml/kg oleic was injected into the tail vein in 4 oleic groups in which the rats were respectively sacrificed after 1h, 2h, 4h, 6h and 24h by exsanguination. In the control group 0.25ml/kg of saline was injected intravenously and sacrificed 2h later. Arterial blood gas, left lung wet/dry ratio, lung index, protein content of BALF, and pulmonary permeability index (PPI) were determined. Fas-L expression of lung tissue was determined by immunohistochemical method and protein expression of PI3-K was determined by Western blot and immunohistochemical method. Results PaO_2 was lowered, and left lung wet/dry ratio, lung index, and protein content of BALF and PPI were increased obviously after oleic acid injection. Fas-L and PI3-K in bronchus epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells were highly expressed in the lung of ALI rats, and peak value of PI3-K appeared earlier than Fas-L. Conclusion Fas-L related apoptosis of bronchial epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells participated in the pathogenesis of earlier period of ALI through PI3-K transduction pathway.
