Expression of heat shock protein 70 and nuclear factor-?B in ventilator-induced lung injury in rabbit
- VernacularTitle:呼吸机所致肺损伤家兔HSP70和NF-?B表达的意义
- Author:
Xuebing QIN
;
Senyang YU
- Publication Type:Journal Article
- Keywords:
respiratory distress syndrome, adult;
heat-shock proteins 70;
NF-kappa B
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate heat shock protein 70 (HSP70) and nuclear factor-?appa B(NF-?B)expression in the lung after mechanical ventilation with PEEP(positive end expiratory pressure)in a rabbit model of ventilator-induced lung injury (VILI). Methods Thirty healthy adult white rabbits were randomly divided into three groups. After anesthesia and tracheostomy were installed, rabbits received ventilation with different tidal volumes for 4 hrs. In PEEP group, rabbits received ventilation withVT=40ml/kg, PEEP=3cmH_2O; group, rabbits received ventilation with VT=40ml/kg, PEEP=0cmH_2O; and NVC group, with normal ventilation with VT=10ml/kg. The different modalities of ventilation were maintained for 4 hours. After 4 hours, rabbits were killed by exsanguination. Lung histopathology was examined and compared among three groups. Expression of HSP70 and NF-?B were determined by using Western-blot technique. Results There were marked pathological changes in the lung tissue in group ZEEP, but pathological changes were relatively milder in group PEEP. After injurious ventilation for 4 hours, there was a significantly higher expression of HSP70 and NF-?B in group ZEEP, while that of NF-?B was negatively correlated with HSP70 levels. There was a significant lower expression of NF-?B in the lung of group PEEP. Conclusion We postulate that HSP70 expression protects the lung against ventilator-induced lung injury by down-regulating expression of NF-?B in the lung. Mechanical ventilation with PEEP can protect the lung against ventilator-induced lung injury through down-regulation of NF-?B in the lung.
