Studies on deduction of the postmortem interval based on the stability of mRNA
- VernacularTitle:基于mRNA稳定性推断死亡时间的研究
- Author:
Fangcheng ZHU
;
Guangmei ZHENG
;
Liang REN
- Publication Type:Journal Article
- Keywords:
RNA stability;
postmortem interval;
glyceraldehyde-3-phosphate dehydrogenase
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study changes in expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the kidney and spleen of dead rats and its application in deducing the postmortem intervals (PMI), so as to look for a new method for PMI deduction. Method 28 rats were killed by cervical dislocation, placed in a chamber with temperature-controlling system at 20℃ for 0 to 48h postmortem, and autopsied at 0, 8, 16, 24, 32, 40 and 48h respectively (n=4 for each time point). The expression of GAPDH mRNA in kidney and spleen was assessed with fluorescence semi-quantitative RT-PCR. Results The amplified products of GAPDH mRNA could be detected in kidney within 40h and in spleen within 32h postmortem respectively, and they showed a decreasing tendency as its fluorescence intensity declined gradually and lost at last. Conclusion Detection of GAPDH mRNA expression in kidney and spleen of rats at different periods after death by fluorescence semi-quantitative RT-PCR seems to be of some value for estimation of PMI.
