Subcellular localization of nuclear factor kappa B in term human fetal membranes and myometrium during labor.
- Author:
Suk Joo CHOI
1
;
Seong O MOON
;
Hyung Sun KIM
;
Soo Young OH
;
Cheong Rae ROH
;
Jong Hwa KIM
Author Information
1. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. jh369.kim@samsung.com
- Publication Type:Original Article
- Keywords:
NF-kappa B;
Myometrium;
Fetal membranes;
Labor
- MeSH:
Amnion;
Animals;
Chorion;
Cytoplasm;
Extraembryonic Membranes*;
Female;
Humans*;
Mice;
Myometrium*;
NF-kappa B*;
Pregnant Women;
Transcription Factor RelA
- From:Korean Journal of Obstetrics and Gynecology
2006;49(1):24-30
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the subcellular localization of nuclear factor kappa B (NF-kappa B) in term human fetal membranes and myometrium during labor. METHODS: Fetal membranes and myometrial tissue were collected from term pregnant women undergoing cesarean delivery after labor (n=4) and before labor (n=4). An immunostaining was done with NF-kappa B p65 antibody. The intensity and distribution of nuclear immunostaining of NF-kappa B p65 subunit were evaluated visually using a semiquantitative analysis. RESULTS: NF-kappa B p65 was present in all tissues studied, and it was localized principally in the cytoplasm of cells of amnion and chorion. But, NF-kappa B p65 was localized more abundant in the nucleus than in the cytoplasm in myometrial cells. In amnion, chorion and myometrium, the staining scores of nuclear NF-kappa B did not show any difference between the after-labor group and before-labor group. CONCLUSION: In human term gestational tissues, subcellular localization of NF-kappa B showed cytoplasmic predominance in amnion and chorion, and nuclear predominance in myometrium. But these subcellular localizations did not change during labor.
