Gene expression of circumsporozoite protein and erythrocyte binding protein of Plasmodium yoelii in the sporozoites from oocyst and salivary gland at different phases
- VernacularTitle:约氏疟原虫卵囊与唾液腺子孢子不同时相点的CSP与MAEBL基因表达研究
- Author:
Jide CHEN
;
Xilin ZHANG
;
Jianhua DUAN
;
Bei SONG
- Publication Type:Journal Article
- Keywords:
circumsporozoite protein;
erythrocyte binding protein;
Plasmodium yoelii;
Anopheles stephensi
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the gene expression change of circumsporozoite protein (CSP) and erythrocyte binding protein (MAEBL) in the Plasmodium yoelii sporozoites from oocyst and salivary gland at different phases during Plasmodium yoelii development in mosquitos. Methods The total RNA of plasmodium was extracted from the abdomen on day 5, 7, 10 and the thoraces on day 12, 15, 18 after Anopheles stephensi was infected by Plasmodium yoelii, then the expression of CSP and MAEBL mRNA was analyzed by semiquantitative RT-PCR with rRNA 28S as control. Results The transcription of CSP and MAEBL of plasmodium sporozoite were obviously up-regulated during the mosquito stage (P
