Expression and significance of multidrug resistance-associated protein 1 in the liver tissues of rat obstructive jaundice models
- VernacularTitle:多药耐药相关蛋白1在梗阻性黄疸大鼠肝组织中的表达及意义
- Author:
Jianyong ZHU
;
Yingguo CHEN
;
Ping BIE
- Publication Type:Journal Article
- Keywords:
obstructive jaundice;
multidrug resistance-associated protein 1
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of multidrug resistance-associated protein 1 (Mrp1) on bilirubin metabolism in rat obstructive jaundiced (OJ) models. Methods Eighty Wistar male rats were randomly divided into three groups: sham operation group in which the common bile duct was educed but not ligated; common bile duct ligation (CBDL) group in which the common bile duct was ligated and cut off; bile reflow group in which on day 14 after common bile duct ligation operation, the internal drainage from common bile duct to duodenum by silica gel duct was performed. Serum prealbumin, serum total bilirubin and urine direct bilirubin were assayed routinely. The expressions of mrp1 mRNA and protein were detected in the liver tissues by semi-quantitative RT-PCR and indirect immunofluorescence respectively. Results Serum prealbumin was descending significantly (P
