Effects of wild-type INK4a gene on radiosensitivity of lung adenocarcinoma cell line A549
- VernacularTitle:野生型INK4a基因转染对肺腺癌A549细胞放疗敏感性的调控
- Author:
Zhonghua CHEN
- Publication Type:Journal Article
- Keywords:
non-small lung carcinoma;
INK4a gene;
transfection;
radiotherapy sensitivity;
cell cycle
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of transfection of plasmid pcDNA3.1-p16 containing the wild-type(wt) INK4a gene on the radiotherapy sensitivity of human lung adenocarcinoma cell line A549. Methods Using cationic liposome, the recombinant eukaryotic expression vectors pcDNA3.1-p16 were introduced into A549 cell line and named as A549-p16, in which the gene site INK4a was lost. By RT-PCR, immunocytochemistry and Western blotting after G418 selection, A549 cells stably expressing p16 were obtained. The parental cell and negative control cell with plasmid pcDNA3-LacZ were used as controls.The colony formation rate and 50% inhibition dose(ID_ 50) were measured and analyzed after radiation. Results As compared with the parental cell and negative control cell, re-expression of p16 in A549 induced the cell arrest at G_ 0/G_ 1. The growth rate and the colony formation rate decreased and the ID_ 50 decreased slightly in A549-p16. Conclusion The exogenous introduction of wtINK4a gene into lung adenocarcinoma cells A549 increased the radiosensitivity.
