Effects of Rutosids on Apoptosis of Retinal Capillary in Rats with Different Courses of Diabetes
- VernacularTitle:络通对不同病程糖尿病大鼠视网膜微血管细胞凋亡的影响
- Author:
Shuiping ZHOU
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
apoptosis;
perithelial cell;
Rutosids
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the effects of Rutosids on apoptosis of retinal capillary in rats with different courses of diabetes.Methods: Diabetes models were prepared by injecting streptozotocin 65mg/kg via rats' abdominal cavity.Diabetes rat models were randomly divided into four groups in terms of diabetes model group(DMG),Rutosids intervention group(RIG),Rutosids therapy group 1(RTG1) and Rutosids therapy group 2(RTG2).Eyeballs were selected separately,retinals digesting stretched preparation capillary samples were prepared,and apoptosis cells were specificity marked by PAS dyeing and ribonucleotide terminal transferase mediating dUTP nick-translation(TUNEL method),to observe below light microscope.Results: Compared with the homeochronous DMG,the apoptosis of retinal pericapillary cells in RIG was not obvious;perithelial cell apoptosis of RTG1 was relatively common,a few endothelial cells were apoptosis;endotheliocyte apoptosis of RTG2 was increased after treating for 3 months,but obviously lighted than 12 months model group.Conclusion: Rutosids has the preferable inhibitory action on perithelial cell apoptosis of early metaphase retinal microangiopathy in diabetes rats.
