Effects of Ropivacaine and Bupivacaine on glutamate-evoked currents in cultured rat hippocampal neurons
- VernacularTitle:罗哌卡因和布比卡因对大鼠海马神经元谷氨酸电流的影响
- Author:
Yuge JIANG
;
Weidong MI
;
Longhe XU
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
Ropivacaine;
Bupivacaine;
patch clamp;
glutamate;
hippocampus
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe and compare the effects of Ropivacaine and Bupivacaine on glutamate-evoked currents in cultured rat hippocampus neurons.METHODS: Rat hippocampal neurons were dissociated and cultured.Glutamate-evoked currents were recorded by whole cell patch clamp recording.Effects of Ropivacaine and Bupivacaine on glutamate-evoked currents were observed.Drugs were given by pressure ejection or applicated in the bath.RESULTS: Glutamate(100(mmol?L~(-1))) can activate inward currents in cultured rat hippocampus neurons and this currents could be locked by non-NMDA antagonists DNQX.At concentrations of 10,50,100(mmol?L~(-1)),both Bupivacaine and Ropivacaine could obviously decrease glutamate-evoked currents in cultured rat hippocampus neurons.At higher concentration of 50 and 100(mmol?L~(-1)),the reduced amplitude of glutamate-evoked currents by Ropivacaine was larger than that of by Bupivacaine(P
