The effect of PA on the proliferation and maturation of cord blood derived DC in vitro
- VernacularTitle:多抗甲素对脐血树突状细胞体外扩增、成熟的影响
- Author:
Huilin GONG
;
Changfu XU
;
Liping MO
;
Jian ZHANG
;
Shuiping HAN
;
Hengli LI
- Publication Type:Journal Article
- Keywords:
polyactin A (PA);
dendritic cell (DC);
proliferation in vitro
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of PA on the proliferation and maturation of cord blood derived DC in vitro. Methods The monocytes were isolated from human umbilical cord blood and cultured with PA, GM-CSF+IL-4+TNF-?(GTI), GTI+PA (GTIP), respectively. On day 7 of cultivation, the CD1a, CD83,HLA-DR and CD34 antigens expression of cells were analyzed by immunohistochemistry. Result The cells with typical morphological properties of DC can be observed with electron microscope among PA, GTI and GTIP groups. The CD1a and CD83 positive rates of PA group increased significantly, reaching 19.63%?3.61% and 14.52%?5.79% respectively, much higher than that of the control. In addition, compared with GIT group, the positive rate in GTIP group has increased remarkably. Conclusion PA does not only promote the proliferation and maturation of cord blood derived DC, but also cooperate with GTI to enhance the production of DC. PA is a useful activator of DC.
