Sequence analysis of muscarinic receptors M1, M3 and M5 expression by cultured rat astrocyte cells
- VernacularTitle:星形胶质细胞毒蕈碱样乙酰胆碱受体M1、M3、M5亚型基因克隆及序列分析
- Author:
Xiaojuan ZHANG
;
Hai WANG
- Publication Type:Journal Article
- Keywords:
non-neuronal cholinergic system;
astrocyte cell;
gene clone;
PCR;
sequence analysis;
muscarinic receptor
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To clone the muscarinic receptors M1, M3 and M5 sequences of astrocyte cells,and compare the gene and protein sequences with those of neurons. Methods Specific primers were designed to clone the M1, M3 and M5 sequences of astrocyte cells by RT-PCR according to those of neurons,then sequenced the sequences. Result By comparing the M1, M3 and M5 sequences expression by astrocyte cells with those by neurons and we found four, eight,and one different bases and one, four and one different amino acids in M1, M3 and M5 between astrocyte cells and neurons respectively. Conclusions The gene and protein sequence differences are evident in M1, M3 and M5 between astrocyte cells and neurons.
