Two-dimensional electrophoresis in proteomic comparison and identification of brain stem proteins in seasickness adaptive and non-adaptive rats
- VernacularTitle:晕船适应与不适应大鼠脑干蛋白质双向电泳图谱的比较与鉴定
- Author:
Bo SU
- Publication Type:Journal Article
- Keywords:
seasickness;
brain stem;
proteome;
two-dimensional electrophoresis
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a two-dimensional eletrophoresis system for total brain stem proteins of seasickness adaptive and non-adaptive rats and to identify the differentially expressed proteins for investigation of the possible mechanism of seasickness adaptation.Methods: According to the kaolin intakes after seasickness stimulation,30 rats were divided into non-seasickness group(n=10),seasickness adaptive group(n=12) and seasickness non-adaptive group(n=8).Another 10 normal rats not receiving stimulation were taken as blank control.The brain stem proteins of seasickness adaptive and seasickness non-adaptive rats(n=6) were isolated by two-dimensional electrophoresis;the differentially expressed proteins were identified by peptide mass fingerprint(PMF).Results: During a 21-day stimulation,the amount of kaolin consumption in seasickness adaptive rats experienced an increasing phase and a subsequent decreasing phase;seasickness nonadaptive rats consumed significantly more kaolin during the whole 21-day stimulation than the blank control rats did(P
