Hepatic gene expression profile early after ~(60)Co-? irradiation in mice
- VernacularTitle:~(60)Co-?照射后早期小鼠肝组织基因表达谱
- Author:
Lihua SONG
- Publication Type:Journal Article
- Keywords:
radiation;
liver;
gene expression profiling
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the hepatic gene expression profile early after ~(60)Co-? irradiation in mice with cDNA microarray,in an attempt to understand the molecular mechanism of irradiation damage to liver in mice.Methods: Total RNA was extracted from mice liver 2 days after irradiation with ~(60)Co-? ray and from normal mice liver.Hepatic gene expression patterns of irradiated and normal mice were compared with cDNA microarray(4 096 genes).RT-PCR was performed to validate the cDNA microarray results.Results: Compared with normal group,124 genes showed a differential expression in irradiation group,with 46 genes upregulated(maily cell cycle and transcription regulation related proteins) and 78 down-regulated(mainly cytoskeletal genes).Of the 124 genes,57 had identified functions and could be divided into 6 groups: DNA repair and stress response,cytoskeleton,hydronium channel/transport protein,signaling transduction,intermediary metabolism,and immune related proteins.RT-PCR analysis indicated that the expression of heat shock 70kD protein 5(Hspa5),RAS p21 protein activator 3(Rasa3),and NAD(P)H dehydrogenase,quinone 1(Nqo1) were consistent with cDNA microarray data.Conclusion: Irradiation-induced hepatic injury is of multi-target and multi-pathway.
