Effects of different n-6/n-3 polyunsaturated fatty acid ratio on lipid metabolic gene expression in breast cancer cell lines
- VernacularTitle:n-6/n-3多不饱和脂肪酸不同比例对乳腺癌细胞脂代谢调控基因的影响
- Author:
Na WEI
;
Mantian MI
- Publication Type:Journal Article
- Keywords:
n-6/n-3 polyunsaturated fatty acid ratio;
breast cancer cell;
lipid metabolic gene;
MAPK activity
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different n-6/n-3 polyunsaturated fatty acid ratio on the lipid metabolic gene expression and MAPK activity in breast cancer cell lines. Methods MCF-7 and MDA-MB-231 cells were treated with n-6 polyunsaturated fatty acid, or n-3 polyunsaturated fatty acid, or the mixture of n-6/n-3 polyunsaturated fatty acid at ratio of 1∶1, 5∶1, 10∶1. The cell proliferation capacity was detected by MTT, the expression of lipid metabolic gene (COX-2, 5-LOX, PPAR? and PPAR?) were assayed by RT-PCR, MAPK phosphorylation levels were detected by Western blotting. Results COX-2 mRNA expression was not found in MCF-7 cells. As compared to normal control group, pure n-6 and 10∶1 n-6/n-3 promoted cell proliferation, up-regulated the expression of COX-2, 5-LOX, PPAR? and PPAR? mRNA , increased MAPK phosphorylation levels in MCF-7 and MDA-MB-231 cells (P
