Transdifferentiation of epidermal stem cells into corneal epithelial cells in vitro
- VernacularTitle:表皮干细胞体外诱导转分化为角膜上皮细胞的实验研究
- Author:
Ke YANG
;
Jin YU
;
Tian YANG
- Publication Type:Journal Article
- Keywords:
epidermal stem cells;
corneal epithelial cells;
transdifferentiation
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the plasticity of transdifferentiation of epidermal stem cells (ESCs) into corneal epithelial cells. Methods Epidermal stem cells enriched by adhering to type Ⅳ collage were induced in vitro by coculture with corneal stromal fibroblasts. The method of HCC were used to show the expression of K12 which is expressed specially in corneal epithelial cells.Results ESCs with greater proliferation potential strongly expressed K19 and ?_ 1 - integrin. After two-week induction, the K12 positive cells could be detected. Conclusion The data suggested that the epidermal stem cells have the potential to transdifferentiate into corneal epithelial cells in vitro.
