Effects of endotoxin preconditioning on inflammatory response after global brain ischemia in rats
- VernacularTitle:内毒素预处理对大鼠全脑缺血/再灌注后炎性反应影响的实验研究
- Author:
Qiang XIANG
;
Liang WEN
;
Qiangguo GAO
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
reperfusion;
endotoxin;
endotoxin
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the protective effects of endotoxin preconditioning on the brain ischemic injury and explore its protective mechanism. Methods Global ischemia for 20 min by four-vessel occlusion model was used in 140 Wistar rats, in which 70 rats were pretreated with MPL at dose of 20 ?g/kg 24 h before global ischemia. Another 10 Wistar rats served as normal controls. Ischemic neurons and neuron density of the hippocampal CA1 region, MPO activity, TNF-? activity, IL-1? activity were assayed at 4, 12, 24, 48, 72, 120, 168 h after reperfusion. Results Pretreatment with endotoxin produced significant reductions in ischemic neuron number, MPO activity, TNF-? activity and IL-1? activity. Conclusion Endotoxin preconditioning protects brain from subsequent ischemic injury, in which the suppression of cellular inflammation may be the protective mechanism.
