Mass spectrometric proteome analyses of plasma obtained from patients suffering from rheumatoid arthritis
- VernacularTitle:双向电泳-质谱法寻找类风湿关节炎疾病相关蛋白
- Author:
Ganping BAI
;
Lina ZHOU
;
Weifeng HE
;
Gaoxing LUO
;
Xiwei CHEN
;
Shaoxuan YI
;
Yongfei FANG
;
Ju WU
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
plasma;
proteome;
two-dimensional electrophoresis
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find the different plasma-associated proteins of rheumatoid arthritis (RA) by using two-dimensional gel electrophoresis for understanding the pathogenesis of RA. Methods The total protein from either RA patients or normal ones was prepared by means of immobilized pH gradient based on two-dimensional gel electrophoresis. After silver staining, gel-image analysis was performed by using PDQuest. The differentially expressed proteins were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). Results 2-DE patterns of plasma from controls and RA patients were presented. The results showed that average number of protein spots was 592 and 563 respectively, and the corresponding average matching rate was 89% and 87% respectively. Gel-image analysis revealed that there were 24 differential protein spots. A total of 15 differential protein spots were successfully identified by MALDI-TOF-MS, of which 6 proteins were up-regulated as compared with control. Conclusion The differentially expressed proteins can be observed in plasma from RA and controls, which can be used to elucidate the pathogenesis of RA for further study.
