Diazoxide increases glutamate uptake by astrocytes via activating mitochondrial ATP-sensitive potassium channels
- VernacularTitle:线粒体K_(ATP)开放剂二氮嗪促进星形胶质细胞摄取谷氨酸
- Author:
Cuiping DAI
- Publication Type:Journal Article
- Keywords:
mitochondrial ATP-sensitive potassium channel;
diazoxide;
astrocyte;
glutamate transporters
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To demonstrate the effect of mitochondrial ATP-sensitive potassium channel opener,Diazoxide,on glutamate uptake activity of astrocyte. METHODS: Primary astrocytic cultures were prepared from cerebral cortices of rats within 3 days old.Glutamate uptake activity of astrocytes was measured using isotopic techniques to detect intracellular -labeled D,L-glutamate concentration.RESULTS: Diazoxide increased -glutamate uptake in a concentration-dependent manner,and could significantly reverse MPP~+-induced glutamate uptake inhibition.The above effects of Diazoxide were blocked by 5-hydroxydecanoate,a selective mitochondrial ATP-sensitive potassium channel blocker.CONCLUSION: Diazoxide increase the activity of glutamate transporters in astrocytes by opening mitochondrial ATP-sensitive potassium channel.
