Sera Pharmacological Effect of Anti-fibrosis Herbs and Selenium on the Proliferation and Apoptosis of Hepatic Stellate Cells
- VernacularTitle:抗纤Ⅰ号和硒药物血清对肝星状细胞增殖和凋亡的影响
- Author:
Yutong HE
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cells;
Chinese herbs;
Selenium;
TGF-?_(1);
NF-?B
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the sera pharmacological effect of anti-fibrosis herbs and selenium on the proliferation and apoptosis of hepatic stellate cells.Methods: The sera from the rats fed by colchicines(Col group),anti-fibrosis herbs(AF group) and anti-fibrosis herbs plus selenium(AS group) acted on the hepatic stellate cells(HSCs).MTT assays and Flow Cytometry were employed to estimate the proliferation of HSCs.The expressions of TGF-?_(1)mRNA were detected by semi-quantified RT-PCR.The activity of NF-?B binding DNA was examined by EMSA.Results: The inhibitive rate of AF group and AS group were much higher than that of Col sera at the concentration of 10%～40%.The proliferation index_()of AF group and AS group was lower than that of Col group statistically.Sera of treatment group could induce the apoptosis of HSCs.The expressions of TGF-?_(1)mRNA in AF and AS group were markedly decreased compared with Col group.The activity of NF-?B binding DNA of AF group,AS group was much lower than that of Col group.Conclusion: These results suggested that anti-fibrosis herbs and selenium may inhibited the proliferation of HSCs and induce apoptosis of HSCs in vitro,which may be related to down-regulation the activity of NF-?B binding DNA and TGF-?_(1) expression.
