Partial nucleotide acid sequence analysis of hepatitis E virus isolated from epidemic outbreak and sporadic patients with hepatitis E in Guangzhou
- VernacularTitle:广州戊型病毒性肝炎暴发株和散发株部分序列比较
- Author:
Guangwen ZHANG
;
Jianguo LI
;
Ting FENG
- Publication Type:Journal Article
- Keywords:
Hepatitis E;
Epidemiology;
Base sequence;
Genotype;
Hepatitis E virus
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotype and gene mutation of hepatitis E virus isolated from the serum and stool samples of the patients with epidemic outbreak hepatitis E in certain recruit barracks of Guangzhou. Methods The reverse transcription polymerase chain reaction (RT-PCR) was performed to amplify the partial ORF2 nucleotide acid sequences of hepatitis E virus isolated from 34 and 46 inpatients with epidemic outbreak and sporadic hepatitis E respectively. The PCR products of the positive samples were cloned and sequenced. Results The 14 strains, including 12 epidemic strains and 2 sporadic strains, were isolated from the total 80 inpatients. The homology of nucleotide acid and amino acid sequences of 12 epidemic outbreak strains is 95.3%～100% and 94.0% ～100% . The homology between epidemic outbreak strains and sporadic strains is 95.3%～99.3% and 94.0%～100%. Compared with the standard different genotypes of HEV, these strains have the highest homology to the Jap1 strain which belongs to genotype Ⅳ, with homology of 96.0%～100%, respectively. Phylogenetic analysis indicated that these the nucleotide acid sequence homology of 92.0%～95.3% and amino acid sequence stains and Jap1 strain share the same cluster. Conclusion[KG1]Epidemic outbreak strains isolated from the patients in recruit barracks of Guangzhou belong to the genotype Ⅳ of HEV and the nucleotide acid sequences had partial mutation.
