Influence on Gene Expression Due to Replicate Heart-Qi Deficiency Syndrome on Wistar Rat Caused by Complex Factor
- VernacularTitle:心气虚证复合因素造模法对Wistar大鼠基因表达的影响
- Author:
Zhiqing CHENG
;
Li YAO
;
Yexia TANG
- Publication Type:Journal Article
- Keywords:
heart-qi deficiency syndrome;
gene expression;
immunological function repression;
vascular endothelium damage;
rats
- From:
Journal of Zhejiang Chinese Medical University
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] Using gene chip to detect the influence on gene expression due to replicate Heart-Qi Deficiency Syndrome on Wistar Rat caused by complex factor including slimming, forced swimming and large dosage of propanolol. [Methods] Every animal in experiment group took food according to 5g/100g body weight, and was forced to swim by taking weight according to its 5% body weight until sank beyond 10 seconds. From 17th day ig propanolol 24mg/Kg and lasted for 4 days. At 21st day we took out hearts and picked up general DNA to prepare probe, then cross with gene chip. [Results] Compared with control group, expression of gene NM-017329,NM-016997 and NM-030850 showed down regulation obviously, expression of gene NM-031345 and NM-024349 showed up-regulation significantly. [Conclusion] Repression of immunological function and damage of vascular endothelium may be the main mechanism resulting in Heart-Qi Deficiency Syndrome.
