Study on Method of Expanding and Culturing Rat Mesenchymal Stem Cells in vitro and Its Biological Characteristics
- VernacularTitle:大鼠骨髓间质干细胞体外培养扩增及生物学特性研究
- Author:
Qin YU
;
Junlan LIAN
;
Yan WANG
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
marrow;
biological characteristics;
rat
- From:
Journal of Zhejiang Chinese Medical University
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a method to seperate rat mesenchymal stem cells(rMSCs) from rat marrow, culture rMSCs in vitro, and study its biological characteristics. [Methods] rMSCs were seperated from rat marrow with wall-sticking method and expanding cultured in vitro. To draw the growth curves of cells at 1th, 3th, 5th passage, detect the surface antigens with flow cytometry. To evaluate the effect of fetal calf serum with different concentrations to rMSCs. [Results] There were approximately (5～6)?105 cells after primary culture and over (2~3)?108 rMSCs at 5th passage. The appearance of rMSCs is like shape of spear and its growth curves are like shape of S. The rMSCs were positive for CD90 and negative for CD45. [Conclusions] Cells seperated and cultured by the method established in this experiment were rMSCs. They have the biological characteristics of rMSCs, and are good resources for study in tissue engineering.
