Construction,expression and examinations of recombiant plasmid with pmRNA IRES-hKDR
- VernacularTitle:pmRNA IRES-hKDR重组质粒的构建、表达及鉴定
- Author:
Xiaoting SHAO
;
Linying SUN
;
Xiuli LIU
;
Chang LIU
;
Xiaohua TAN
- Publication Type:Journal Article
- Keywords:
KDR,recombiant plasmid;
Gene therapy
- From:
Chinese Journal of Primary Medicine and Pharmacy
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct recobinant pmRNA IRES-hKDR and lay a primary foundation for further study of gene therapy for tumors.Methods pmRNA IRES-hKDR was obtained from pDC520 hkDR and pmRNA IRES-Luc which was reserved by our laboratory through molecular biology technology and was transfected into Hepall-6 via lipsome after identification of PCR,restriction enzyme digesting and DNA ELISA and Western blot.Results The recombiant plasmid had pmRNA IRES-hKD gene which was expressed in Hepall-6//G418 in vitro and the expressed protein was secreted out of the cells could response with specific hKDR antibody,which was identified by Western blot.Conclusion An eukaryotic expression recombiant plasmid pmRNA IRES-hKDR can be constructed successfully which provides the possibility of further researches on its anti-tumor effect.
