Study on metabolites of scutellarin in gastrointestinal tract by UPLC-MS/MS method
- VernacularTitle:UPLC-MS/MS联用法分析灯盏花乙素在胃肠道的代谢物
- Author:
Wenzheng JU
- Publication Type:Journal Article
- Keywords:
scutellarin;
UPLC-MS/MS;
acid hydrolysis;
?-glucuronidase;
intestinal bacteria metabolism
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The metabolites of scutellarin were studied in gastrointestinal tract.METHODS: Scutellarin was hydrolyzed with hydrochloric acid and ?-glucuronidase.Human intestinal flora and scutellarin were incubated in vitro.The metabolites were identified by UPLC-MS/MS method.RESULTS: Scutellarin could be hydrolyzed with hydrochloric acid and ?-glucuronidase,and could be metabolized by human intestinal bacteria.A main metabolite,scutellarein,also was found in the plasma of healthy volunteers after oral administration.CONCLUSION: There were scutellarin and scutellarein in intestinal before absorption.It was suitable for scutellarin pharmacokinetic studies to determine scutellarein or total aglycone.
