Modified retinal vascular digest preparations combined with laser scanning confocal microscope-a three-dimensional exami nation method for retinal vessels
- VernacularTitle:改良的视网膜血管消化铺片联合共聚焦显微镜观察-视网膜血管的三维检查方法
- Author:
Yan CUI
;
Xun XU
;
Qing GU
- Publication Type:Journal Article
- Keywords:
retinal vessels;
digest preparations;
microscopy, confocal
- From:
Ophthalmology in China
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a three-dimensional examination method for retinal vessels. Design Experimental study. Participants Retinal vascular digest preparations of rat. Methods After deep anaesthesia, rats were sacrificed and perfused till eyeballs pale. Then 4% paraformaldehyde was perfused for vascular internal fixation. Eyeballs were fixed in 4% paraformaldehyde for 24hr, and the retinal vessels were isolated with collagenase digest technique. After immunofluorescence staining, the retinal vessels were observed with laser scanning confocal microscope. Main Outcome Measures The digest state of retinal vascular digest preparations and three-dimensional observation of the preparations. Results Ideal complete retinal vascular digest preparations were obtained after collagenase digestion. Three-dimensional characteristics of retinal vessels can be observed with laser scanning confocal microscope. Conclusions Modified retinal vascular digest preparations combined with laser scanning confocal microscope provided us a method for retinal vascular three-dimensional structure observation.(phthalmol CHN,2006,15:138-141)
