CTLA-4Ig combined with ICAM-1 mAb promotes immune tolerance induced by donorderived immature dendritic cells in recipient mice
- VernacularTitle:CTLA-4Ig与ICAM-1单抗促进供者来源的未成熟树突状细胞诱导移植受体免疫耐受
- Author:
Guoshan DING
- Publication Type:Journal Article
- Keywords:
cytotoxic T lymphocyte-associated antigen-4-Ig;
intercellular adhesion molecule-1;
antibodies,monoclonal;
dendritic cells;
transplantation tolerance
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of CTIL-4Ig combined with Anti-ICAM-1 mAb in promoting immune tolerance induced by donor-derived immature dendritic cells(imDC) in recipient mice.Methods: Male mice were divided into 4 groups: control group(receiving only imDC),CTLA-4Ig group,ICAM-1 mAb group and CTLA-4Ig+ICAM-1 group.Mice were transfused with donor-derived imDC 7 days before they received heart transplantation in company with daily injection of ICAM-1 mAb,CTLA-4Ig or both for the following 2 weeks.Immunological analysis was performed in mice 7 days and 21 days after heart transplantation.Results: CTLA-4Ig alone or in combination with ICAM-1 mAb significantly inhibited T cells proliferation to alloantigen stimulation,impaired lymphocyte cytotoxicity,suppressed production of IL-2,IFN-? by Th1,increased production of IL-10,and obviously decreased the production of alloantibody IgG in recipient mice treated with donor-derived imDC.ICAM-1 mAb alone had no significant effects on T cells proliferation and production of Th-derived cytokines except for IL-2.Conclusion: ICAM-1 mAb combined with CTLA-4Ig can enhance immune tolerance induced by donor-derived imDC in recipient mice through induction of T cells hypo-responsiveness,inhibition of lymphocyte cytotoxicity and B cell immunoreation,and promotion of Th2 polarization in vivo.
