Inhibitory effects of ?-lipoic acid on cell proliferation and ICAM-1 expression induced by high glucose in rat mesangial cells
- VernacularTitle:?-硫辛酸抑制高糖诱导的系膜细胞增殖及细胞间黏附分子1的表达
- Author:
Hui LI
- Publication Type:Journal Article
- Keywords:
?-lipoic acid;
intercellular adhesion molecule-1;
mesangial cells;
cell proliferation;
high glucose
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the effects of ?-lipoic acid on cell proliferation and expression of intercellular adhesion molecule1(ICAM-1) induced by high glucose(HG) in rat mesangial cells(MCs).Methods: Rat mesangial cells were co-cultured with normal glucose(5.6 mmol/L,NG group),high glucose(25 mmol/L,HG group),and HG+?-lipoic acid(50,100,200,300 ?mol/L)for 12,24 and 48 h,respectively.Cell proliferation was assessed by MTT;the levels of ICAM-1 in the supernatants were determined by enzyme-linked immunosorbent assay(ELISA);and ICAM-1 mRNA expression was measured by semi-quantitative RT-PCR.Results: ?-lipoic acid at 50-300 ?mol/L inhibited the proliferation of MCs.Semi-quantitative RT-PCR showed that ?-lipoic acid(100 and 200 ?mol/L)significantly inhibited the high glucose-induced increase of ICAM-1 mRNA((P
