Study on enrichment and purification of astragalosides Ⅳ from Radix astragali with macroporous resins
- VernacularTitle:大孔树脂吸附法对黄芪皂苷Ⅳ富集纯化工艺的研究
- Author:
Yue GAO
- Publication Type:Journal Article
- Keywords:
Radix astragali;
astragaloside Ⅳ;
macroporous resin;
chromatography,high performance liquid
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the enrichment and purification method of astragaloside Ⅳ from Radix astragali with macroporous resin,so as to lay a foundation for the large scale production of astragaloside Ⅳ.Methods: A HPLC-MSD method was established to determine the content of astragaloside Ⅳin Radix astragali.The adsorption ability and capability of different macroporous resins were investigated.The optimal alcohol concentration and capability of alcohol for elution were also confirmed through the determination of astragaloside Ⅳ in the eluted solution.Results: HPLC-MSD achieved satisfactory results in determination of astragaloside Ⅳ.D101 was proven to be the most potent macroporous resin;its adsorption capability of astragaloside Ⅳ was 0.42 mg/g when the elution solution was 5 folds of 70% alcohol.Conclusion: D101 macroporous resin can be used to effectively enrich and purify astragaloside Ⅳ,laying a foundation for analyzing the constituents of total astragalosides.
