Ginkgo biloba extract attenuates advanced glycation end products-induced TGF-?_1,CTGF mRNA expression and oxidative stress in NRK-49F cells
- VernacularTitle:银杏叶提取物对肾间质成纤维细胞中AGEs诱导的TGF-?_1CTGF表达及氧化应激的抑制作用
- Author:
Haidong YAN
;
Xuezhu LI
;
Man LI
- Publication Type:Journal Article
- Keywords:
Advanced glycation end-products;
Extract of Ginkgo biloba;
Renal fibrosis;
Oxidative stress
- From:
Chinese Journal of Practical Internal Medicine
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effects of Ginkgo biloba extract on advanced glycation end products-induced TGF-?_1,CTGF mRNA expression and oxidative stress in cultured NRK-49F cells.Methods From Jan.to Jul.2005,NRK-49F cells were incubated with serum-free medium with or without 25 mmol/L glucose as control.Cells were incubated with medium containing AGEs-BSA with or without 25 mmol/L glucose for 24 h.Cells were pretreated with EGb(50,100mg?mL -1)for 24 h to evaluate effects of EGb.And then intracellular ROS generation was measured by flowcytometry,expression of TGF-?_1 and CTGF mRNA were performed by a semi-quantitative RT-PCR technique.Results Compared with control,AGEs significantly increased the expression of TGF-?_1 and CTGF mRNA and intercellular ROS generation in NRK-49F cells.Incubation with both AGEs and high glucose(25 mmol/L)can upregulate TGF-?_1 and CTGF mRNA expression and intercellular ROS generation,compared with AGEs and high glucose(25 mmol/L)respectively.Preincubation with EGb attenuated AGEs-induced TGF-?_1 and CTGF mRNA overexpression and intercellular ROS generation.Conclusion Attenuation of AGEs-induced TGF-?_1 and CTGF mRNA overexpression in renal fibroblasts through scavenging of free radical and inhibition of oxidative stress may be one of mechanisms underlying EGb in prevention and therapy of DN.
