Inhibition of sialic acid related substances on Pseudomonas aeruginosa infection in rat trachea vulnerated by hydrochloric acid
- VernacularTitle:唾液酸相关物质对盐酸致伤大鼠气管铜绿假单胞菌感染的抑制作用
- Author:
Yong HUANG
;
Guisheng QIAN
;
Shungui XU
- Publication Type:Journal Article
- Keywords:
sialic acid related substrances;
rat;
trachea;
Pseudomonas aeruginose infection
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether sialic acid disaccharides, specificity agglutinins and neuramidinases could inhibit Pseudomonas aeruginose infection in rat trachea vulnerated by hydrochloric acid. Methods One hundred and forty male Wistar rats were vulnerated by hydrochloric acid through intratracheal instillation and then inoculated by Pseudomonas aeruginose admixed Sial ?2,3-Gal, Sial ?2,6-GalNAc, Maackia amurensis agglutinin, Sambucus nigra agglutinin, specificity ?2,3-neuramidinase or non-specificity neuramidinase respectively. Thirty minutes and 48 h after inoculation, whole trachea of 8 rats in each group was weighed, homogenated, and quantitative bacterial culture was performed while whole trachea of another 2 was fixed in 10% methanol, embedded with paraffin and HE stained. Results Sial ?2,3-related disaccharide, agglutinin and neuramidinases could decrease colony count of trachea 30 min after inoculation remarkably. But only Sial ?2,3-Gal and neuramidinases could obviously decrease colony count 48 h later. Sial ?2,6-related substrances have not analogical effects. Conclusion Sial ?2,3-related substrances could interfere Pseudomonas aeruginose binding/multiplication in the trachea, especially Sial ?2,3-Gal and neuramidinase.
