Apoptosis of microglia and neurons in rats following hazardous fast decompression simulating escape from submerged submarine
- VernacularTitle:潜艇脱险中极快速减压后大鼠小胶质细胞与神经元凋亡的变化
- Author:
Ruiyong CHEN
;
Jingchang LIU
;
Min ZHAO
- Publication Type:Journal Article
- Keywords:
decompressing sickness;
decompressing, explosive;
microglia;
neurons;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of apoptosis of microglia and neuron in adult rats following fast decompression. Methods A model of decompression sickness in rat was reproduced by exposure to 1MPa 5.5min followed by rapid decompression (50s). Brain tissues were collected at 0h, 6h, 24h, 48h and 72h after decompression. The microglia were examined after histochemical staining with FITC-conjugated Isolectin-B4. Cell apoptosis was detected by in situ end labeling TUNEL methods. Results A few IB4-positive microglia could be seen in the brain tissue collected 6h after decompression, and the number of IB4-positive microglia was greatest at 24h (P
