The influence on adhesion between laterally spreading tumor cell and collagen Ⅰ mediated by membrane galectin-1 monoclonal antibody inhibition
- VernacularTitle:细胞膜表面Galectin-1抗体阻断对大肠侧向发育型肿瘤细胞与Ⅰ型胶原黏附的影响
- Author:
Huasheng TONG
;
Yali ZHANG
;
Bo JIANG
- Publication Type:Journal Article
- Keywords:
antibodies, galectin-1;
collagen type Ⅰ;
colorectal neoplasms;
cell adhesion
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence on adhesion between LST-R1 cell and collagen Ⅰ mediated by galectin-1 antibody inhibition. Methods Laser confocal scanning microscopy was employed to detect the expression of galectin-1 on LST-R1 cell membrane.LST-R1 cells inhibited by galectin-1 antibody or not were seeded in the 48-well plate coated with collagen Ⅰ (20?g/well), and the morphology of the adhesive LST-R1 cells was observed and the adhesive cells were counted. Results Laser confocal scanning microscopy showed the expression of galectin-1 on the membrane of LST-R1 cells.The binding rate between galectin-1 and its antibody was 38.2%?0.92%. In the galectin-1 antibody inhibiting group,more and more irregular angular adhesive cells appeared, and most of the adhesive cells were growing in singles, while the adhesive cells,mainly clustered, in the control group were round or roudish. The numbers of adhesive LST-R1 cells in the antibody inhibiting group and the control group were 40 4 and 30 4 respectively (P
